diff --git a/eng/pack/Microsoft.Azure.Functions.V4.PythonWorker.nuspec b/eng/pack/Microsoft.Azure.Functions.V4.PythonWorker.nuspec
index 6531c5df..08afd50f 100644
--- a/eng/pack/Microsoft.Azure.Functions.V4.PythonWorker.nuspec
+++ b/eng/pack/Microsoft.Azure.Functions.V4.PythonWorker.nuspec
@@ -10,12 +10,6 @@
     <copyright>© .NET Foundation. All rights reserved.</copyright>
   </metadata>
   <files>
-    <file src="..\..\3.9_WINDOWS_X64\**" target="tools\3.9\WINDOWS\X64" />
-    <file src="..\..\3.9_WINDOWS_X86\**" target="tools\3.9\WINDOWS\X86" />
-    <file src="..\..\3.9_LINUX_X64\**" target="tools\3.9\LINUX\X64" />
-    <file src="..\..\3.9_OSX_X64\**" target="tools\3.9\OSX\X64" />
-    <file src="..\..\3.9_OSX_ARM64\**" target="tools\3.9\OSX\Arm64" />
-    <file src="..\..\3.9_LINUX_ARM64\**" target="tools\3.9\LINUX\Arm64" />
     <file src="..\..\3.10_WINDOWS_X64\**" target="tools\3.10\WINDOWS\X64" />
     <file src="..\..\3.10_WINDOWS_X86\**" target="tools\3.10\WINDOWS\X86" />
     <file src="..\..\3.10_LINUX_X64\**" target="tools\3.10\LINUX\X64" />
diff --git a/eng/templates/jobs/ci-dependency-check.yml b/eng/templates/jobs/ci-dependency-check.yml
index b3b4cda1..75a9a3dd 100644
--- a/eng/templates/jobs/ci-dependency-check.yml
+++ b/eng/templates/jobs/ci-dependency-check.yml
@@ -9,8 +9,6 @@ jobs:
 
     strategy:
       matrix:
-        Python39:
-          PYTHON_VERSION: '3.9'
         Python310:
           PYTHON_VERSION: '3.10'
         Python311:
diff --git a/eng/templates/jobs/ci-emulator-tests.yml b/eng/templates/jobs/ci-emulator-tests.yml
index 0228f9dc..99efa33d 100644
--- a/eng/templates/jobs/ci-emulator-tests.yml
+++ b/eng/templates/jobs/ci-emulator-tests.yml
@@ -12,8 +12,6 @@ jobs:
 
     strategy:
       matrix:
-        Python39:
-          PYTHON_VERSION: '3.9'
         Python310:
           PYTHON_VERSION: '3.10'
         Python311:
diff --git a/eng/templates/jobs/ci-unit-tests.yml b/eng/templates/jobs/ci-unit-tests.yml
index e035b1a7..9372cb34 100644
--- a/eng/templates/jobs/ci-unit-tests.yml
+++ b/eng/templates/jobs/ci-unit-tests.yml
@@ -12,8 +12,6 @@ jobs:
     
     strategy:
       matrix:
-        Python39:
-          PYTHON_VERSION: '3.9'
         Python310:
           PYTHON_VERSION: '3.10'
         Python311:
diff --git a/eng/templates/official/jobs/build-artifacts.yml b/eng/templates/official/jobs/build-artifacts.yml
index cec31407..8923ac42 100644
--- a/eng/templates/official/jobs/build-artifacts.yml
+++ b/eng/templates/official/jobs/build-artifacts.yml
@@ -7,9 +7,6 @@ jobs:
     os: windows
   strategy:
     matrix:
-      Python39V4:
-        pythonVersion: '3.9'
-        normalizedPythonVersion: '3.9'
       Python310V4:
         pythonVersion: '3.10'
         normalizedPythonVersion: '3.10'
@@ -46,9 +43,6 @@ jobs:
     os: windows
   strategy:
     matrix:
-      Python39V4:
-        pythonVersion: '3.9'
-        normalizedPythonVersion: '3.9'
       Python310V4:
         pythonVersion: '3.10'
         normalizedPythonVersion: '3.10'
@@ -85,9 +79,6 @@ jobs:
     os: linux
   strategy:
     matrix:
-      Python39V4:
-        pythonVersion: '3.9'
-        normalizedPythonVersion: '3.9'
       Python310V4:
         pythonVersion: '3.10'
         normalizedPythonVersion: '3.10'
@@ -123,9 +114,6 @@ jobs:
     os: macOS
   strategy:
     matrix:
-      Python39V4:
-        pythonVersion: '3.9'
-        normalizedPythonVersion: '3.9'
       Python310V4:
         pythonVersion: '3.10'
         normalizedPythonVersion: '3.10'
@@ -161,9 +149,6 @@ jobs:
     os: macOS
   strategy:
     matrix:
-      Python39V4:
-        pythonVersion: '3.9'
-        normalizedPythonVersion: '3.9'
       Python310V4:
         pythonVersion: '3.10'
         normalizedPythonVersion: '3.10'
@@ -199,9 +184,6 @@ jobs:
     os: linux
   strategy:
     matrix:
-      Python39V4:
-        pythonVersion: '3.9'
-        normalizedPythonVersion: '3.9'
       Python310V4:
         pythonVersion: '3.10'
         normalizedPythonVersion: '3.10'
diff --git a/eng/templates/official/jobs/ci-docker-dedicated-tests.yml b/eng/templates/official/jobs/ci-docker-dedicated-tests.yml
index d1376275..ba25ec20 100644
--- a/eng/templates/official/jobs/ci-docker-dedicated-tests.yml
+++ b/eng/templates/official/jobs/ci-docker-dedicated-tests.yml
@@ -12,12 +12,6 @@ jobs:
     
     strategy:
       matrix:
-        Python39:
-          PYTHON_VERSION: '3.9'
-          STORAGE_CONNECTION: $(LinuxStorageConnectionString39)
-          SQL_CONNECTION: $(LinuxSqlConnectionString39)
-          EVENTGRID_URI: $(LinuxEventGridTopicUriString39)
-          EVENTGRID_CONNECTION: $(LinuxEventGridConnectionKeyString39)
         Python310:
           PYTHON_VERSION: '3.10'
           STORAGE_CONNECTION: $(LinuxStorageConnectionString310)
diff --git a/eng/templates/official/jobs/ci-e2e-tests.yml b/eng/templates/official/jobs/ci-e2e-tests.yml
index bf4b8f1e..213975b8 100644
--- a/eng/templates/official/jobs/ci-e2e-tests.yml
+++ b/eng/templates/official/jobs/ci-e2e-tests.yml
@@ -13,12 +13,6 @@ jobs:
 
     strategy:
       matrix:
-        Python39:
-          PYTHON_VERSION: '3.9'
-          STORAGE_CONNECTION: $(LinuxStorageConnectionString39)
-          SQL_CONNECTION: $(LinuxSqlConnectionString39)
-          EVENTGRID_URI: $(LinuxEventGridTopicUriString39)
-          EVENTGRID_CONNECTION: $(LinuxEventGridConnectionKeyString39)
         Python310:
           PYTHON_VERSION: '3.10'
           STORAGE_CONNECTION: $(LinuxStorageConnectionString310)